(Solved): A researcher treats a DNA sample and a plasmid with the \( a m p^{\mathrm{R}} \) and \( l a c Z \) ...

A researcher treats a DNA sample and a plasmid with the \( a m p^{\mathrm{R}} \) and \( l a c Z \) (for the enzyme \( \beta \)-galactosidase) genes with the restriction enzyme EcoRI with the goal of making recombinant DNA molecules. The researcher places the lacZ gene in the cloning region so that it is disrupted by DNA inserts. Place the remaining steps for this process in the correct order. Not all steps will be used. Blue colonies that survive on the culture medium contain the recombinant plasmid. Grow cells containing the recombinant plasmid in a medium with ampicillin and X-gal (a galactose derivative). All the fragments have the sticky ends \( 5^{\prime}- \) GAATTC \( -3^{\prime} \). Remove colonies containing the recombinant plasmid from the culture medium and continue growing. All the fragments have identical sticky ends: \( 5^{\prime} \)-AATT- \( 3^{\prime} \). White colonies that survive on the culture medium contain the recombinant plasmid. Use DNA ligase to join the sticky ends of the DNA sample and the plasmid.