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(Solved): Below is a list of restriction sites and the enzymes that cut them. The cut sites are labeled with t ...
Below is a list of restriction sites and the enzymes that cut them. The cut sites are labeled with
triangles.
EcoRI 5^(')dots G'AA T T C .... \deg
3\deg ... CTTTAAG...5'
5\deg dots GAGCT"C dots3^(. )
3\deg dotsC_(A) TCGAG dots5^(')
5\deg dotsCAA^(T)A T G ... 3*
3\deg ... G TATAC...5 5\deg
5\deg dotsA^(V) C GCG T ... 3\deg
MluI
Spel
5\deg dotsA^(V)C T A G T .... 3^(')
3\deg .. TGATCAA... 5^(')
,5^(')dots GCGGCCGC...3'
Notl
3^(')dots.CGCCGGCG
,5^(')dotsT^(TT) CTAGA A3^(')
Xbal 3^(')dots AGATCA
a. Which enzyme(s) yields the LONGEST cohesive (sticky) ends? (2 pts)
b. Which two enzymes yield cohesive ends that could potentially anneal to each other, even
though the enzymes are different? (4 pts)
c. Trick question: hich enzyme's restriction site is the least likely to be found in a genome? (2 pts)
d. WHY? (4 pts) trick question.