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(Solved): Biochemistry Problems: In the lab you have a solution of 8m(g)/(m)L BSA. How would you use this sol ...



Biochemistry Problems: In the lab you have a solution of

8m(g)/(m)L

BSA. How would you use this solu- tion to make a sample with

6\mu g

BSA in water with a total volume of

6\mu L

? You may use a p1000 pipette (range

100-1000\mu L

) and a p1o pipette (range

0.5-10\mu L

). (Answer with all workings + units) Figure 3: Absorbance was measured at

280nm

in a spectrophotometer over a range of pro- tein concentrations. Three sections of the data are labelled with blue boxes numbered 1,2 , and 3 .

(10%)

Figure 3 shows absorbance at

280nm

for a range of protein concentrations. Explain the results shown in (a) Box 1 (b) Box 2 (c) Box 3 (Answer with approx. 1-2 sentences for each of the above sub-questions)



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