Biochemistry Problems: In the lab you have a solution of
8m(g)/(m)LBSA. How would you use this solu- tion to make a sample with
6\mu gBSA in water with a total volume of
6\mu L? You may use a p1000 pipette (range
100-1000\mu L) and a p1o pipette (range
0.5-10\mu L). (Answer with all workings + units) Figure 3: Absorbance was measured at
280nmin a spectrophotometer over a range of pro- tein concentrations. Three sections of the data are labelled with blue boxes numbered 1,2 , and 3 .
(10%)Figure 3 shows absorbance at
280nmfor a range of protein concentrations. Explain the results shown in (a) Box 1 (b) Box 2 (c) Box 3 (Answer with approx. 1-2 sentences for each of the above sub-questions)
