Creation of a transgenic mouse line in which a tumor suppressor gene is conditionally knocked out involves the following steps (choose all that apply). The vector is injected into differentiated pre-cancerous cells. The vector containing the TSG and LoxP sites is electroporated into embryonic stem cells.* The targeting vector containing the tumor suppressor gene is constructed with LoxP target sites* TSG+/fl mice are crossed with mice containing a tissue-specific Cre or Cre-ER to allow for conditional knockout of the TSG.* TSG+/+ mice are crossed with TSG+/fl mice to generate TSGfl/fl mice