ou believe that one or both of these motor neurons may be responsible for activating the muscles involved in the process of "singing" wing vibration that the male flies perform during courtship. To test this hypothesis you have developed four GAL4 drivers: AN12GAL4, SN45GAL4, mN78GAL4, and mN90GAL4, each specifically expressed in the neurons AN12, SN45, mN78, and mN90, respectively. Additionally, you have two UAS constructs: UASTetx and UASTRP. You collect male D. melanogaster flies with different GAL4 driver UAS combinations and assess whether the wings vibrate upon exposure to D. melanogaster (m) female pheromone or D. simulans (s) female pheromone (as shown in the table below).
Condition
Pheromone
GAL4 driver
UAS construct
1
m
AN12GAL4
UASTetx
2
s
AN12GAL4
UASTetx
3
m
AN12GAL4
UASTRP
4
s
AN12GAL4
UASTRP
5
m
SN45GAL4
UASTetx
6
s
SN45GAL4
UASTetx
7
m
SN45GAL4
UASTRP
8
s
SN45GAL4
UASTRP
9
m
mN78GAL4
UASTetx
10
s
mN78GAL4
UASTetx
11
m
mN78GAL4
UASTRP
12
s
mN78GAL4
UASTRP
13
m
mN90GAL4
UASTetx
14
s
mN90GAL4
UASTetx
15
m
mN90GAL4
UASTRP
16
s
mN90GAL4
UASTRP
In which conditions above do you expect to see the wing vibrate if neither mN78 or mN90 need to be activated to cause "singing"?
A 1, 2, 5, 6, 9, 13
B 3, 4, 7, 8, 11, 12, 13, 15
C 3, 4, 5, 7, 9, 11, 15, 16
D 3, 4, 7, 11, 15
E 1, 3, 5, 7, 9, 11, 13, 15 (input just the letter option above in the blank below)
In which conditions above do you expect to see the wing vibrate if both mN78 and mN90 must be activated to cause the "singing" behaviour?
A 1, 2, 5, 6, 9, 13
B 3, 4, 7, 8, 11, 12, 13, 15
C 3, 4, 5, 7, 9, 11, 15, 16
D 3, 4, 7, 11, 15
E 1, 3, 5, 7, 9, 11, 13, 15 (input just the letter option above in the blank below)
In which conditions above do you expect to see the wing vibrate if only mN78 needs to be activated to cause "singing"?
A 1, 2, 5, 6, 9, 13
B 3, 4, 7, 8, 11, 12, 13, 15
C 3, 4, 5, 7, 9, 11, 15, 16
D 3, 4, 7, 11, 15
E 1, 3, 5, 7, 9, 11, 13, 15 (input just the letter option above in the blank below)