The isoelectric point, pI, of the protein chymotrypsinogen A is 9.1 , while that of 3a hydroxysteroid dehydrogenase is 6.1 .
What is the net charge of chymotrypsinogen A at pH 5.1 ?
positivenegative
What is the net charge of 3a hydroxysteroid dehydrogenase at pH 7.3 ?
positivenegative
The isoelectric point of serine is 5.68 ; glutamine , 5.65 .
During paper electrophoresis at pH 7.3 , toward which electrode does serine migrate?
positivenegative
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During paper electrophoresis at pH 5.1 , toward which electrode does glutamine migrate