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(Solved): Please answer for how to how to calculate pi values as shown in the picture with diagram then how to ...
Please answer for how to how to calculate pi values as shown in the picture with diagram then how to calculate charge for one alpha-amino group, then charge for one alpha-carboxyl group and charges for all the ionisable r- groups for histidine glycine and aspartate acid plz see the examples and pka values provided. plz give clear formatting and steps
Fill out the related information for all three amino acids in the Pre-lab Chart (Table A). On a
separate piece of paper provide calculations for all pl values; charge for one \alpha -amino group; charge
for one \alpha -carboxyl group and the charges for all the ionizable R-groups. Your typed calculations will
be checked in lab by a TA and are also required to be submitted with your report.
Table A: Expected Properties and behaviors of amino acids
The following shows the steps involved for determining the pl value for Lysine:
Draw the progressive deprotonation of Lvsine:
Find the species that has a net neutral charge (zwitterion species):
Identify the functional groups (and their respective pKa values)
eprotonated before and
after the presence of the zwitterion species:
To determine the pl value, calculate the average of the two pKa values:
pI value for Lysine =(9.0+10.5)/(2)=9.75
