Question 5. Design a diagnostic PCR test for Respiratory syncytial virus (RSV) infection. Viral DNA sequence Tasks: Design two 10-base pair long primers; circle where they will bind (above), write exact DNA sequences, and label 5' ends (below). Calculate their "rule of thumb" Tm. Propose the temps and times for use in a PCR experiment. Indicate the predicted product length from successful amplification and draw a predicted gel "Figure 1" with a MW ladder (using the box below). Primer Primer TmTm PCR cycle (temp/times) PCR product length Figure 1:
