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(Solved): The following experiment was conducted (data shown below): A protease was presented with a variety ...



The following experiment was conducted (data shown below):
A protease was presented with a variety of different peptide subst

The following experiment was conducted (data shown below): A protease was presented with a variety of different peptide substrates that differ only in the C-terminal amino acid (shown in red), which is cleaved. The Kcat and KM was determined for each substrate and a pH 7 buffer was used. Which type of terminal amino acid does this protease cleave most efficiently? Substrate DVSA F DVSA L DVSA E DVSA K DVSA S KM (MM) Kcat (S-1) 10 20 1 10 5 1 2 20 20 10 A hydrophobic aliphatic terminal amino acid A positively charged terminal amino acid A polar uncharged terminal amino acid A hydrophobic aromatic terminal amino acid A negatively charged terminal amino acid


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Answer : The above given experiment was conducted (data shown as above). A protease was presented with a variety of different peptide substrates that differ only in the C - terminal amino acid (shown in red), which is cleaved. The kcat and kM was det
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