(Solved): The following sequence contains the coding strand of a gene and some surrounding genomic DNA. The g ...

The following sequence contains the coding strand of a gene and some surrounding genomic DNA. The gene consists of three exons with two introns. Intron sequences are marked by the inclusion of red square brackets (e.g. AC[ACTG]TG ) in the sequence. Use this sequence to answer questions 8 and 9. 9. Write the mature mRNA sequence: Include the $$5^{?}$$ cap, splice out the introns, and include 20 bases of a polyA tail. To find out where to correctly add the polyA tail, see the information below about the polA signal sequence. Make sure you label the $$5^{?}$$ and $$3^{?}$$ ends of the RNA. 10. RNA Transcription does not require as many proteins as DNA replication, even though they both use Polymerases. Why doesn't transcription require: a. DNA Ligase? b. Single Stranded Binding Protein? c. Topoisomerase (Gyrase)