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Function: require_once
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Function: require_once
We want to sequence the pathogenic E. coli strain O157:H7 that has the genome size of 5.44 Mbp (Megabase pairs = million base pairs). We have access to an instrument that can be tuned for both: how many reads it produces and how long each read is. We will need 150bp long reads to ensure a 10X coverage over the entire genome. At this coverage level, theoretically speaking, bases of the genome will not have been sequenced? Please round to the most appropriate integer. Hint 1: For rounding at the end - think of the biological reality. For example, if you calculate that you need 100.3 reads of 150bp to ensure a 10X coverage, then you should round *up* because you need that full extra read (101) to ensure 10X coverage. If you only sequenced 100 reads, you would necessarily get lower than 10X coverage.